Several studies have shown that UV radiation on the skin results in the formation of reactive oxygen species (ROS) that interact withproteins, lipids and DNA, thus altering cellular functions. The epidermis is composed mainly of keratinocytes, rich in ROS detoxifyingenzymes and in low-molecular-mass antioxidant molecules. However, the increased generation of ROS can overwhelm the naturaldefences against oxidative stress. Therefore treatment of the skin with products containing plant-derived antioxidant ingredients may be auseful strategy for the prevention of UV-mediated cutaneous damage. In the present study we have investigated the in vitro capability ofa Jacquez grapes wine extract (containing a signihydroxycinnamic acids; JW-E), to protect skin against UVB-induced oxidative damage by using a three-dimensional tissue culture modelof human epidermis. The endpoints of our experiments were cell viability, release of interleukin-1mediators of cutaneous ingroups (derived by the oxidative damage respectively of lipids and proteins) and tissue redox balance (expressed by the levels ofreduced glutathione, oxidized glutathione, glutathione peroxidase and glutathione reductase). Taken together, our that the JW-E is an epotential promising candidate as a skin photoprotective agent.Wcant level of proanthocyanidins, together with lower amounts of anthocyanins and and prostaglandin E2 (well-knownXammatory processes), accumulation in the epidermis of malondialdehyde/4-hydroxynonenal and protein carbonylWndings demonstrateYcient botanical mixture able to prevent skin oxidative damage induced by UV-B exposure and may thus be a