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Oesophageal irritation
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CONTEXT
Similarly to all other non-validated tests, users are completely free to develop their own protocol and thus to get test perfectly adapted to their specific needs. This includes thus a multitude of possibility regarding protocol steps (pre-incubation, application, rinsing…) and used endpoints (biochemistry, molecular biology, OMICS, cell imaging...). Moreover the main advantage is the possibility to use well-known references as positive and negative control that may be different from regulatory toxicological ones.
DESCRIPTION
Example of applications:
- Safety assays:
- profiling of potential local tolerance issues from developed compounds or products after oral administration on the oesophagus
- Efficacy assays:
- Genomic and transcriptomic signature of compounds for the treatment of Candidosis or Barret's disease (reflux)
MODELS
REFERENCES
Influence of voriconazole and fluconazole on reconstituted multilayered oesophageal epithelium infected by Candida albicans. J.Bernhardt, H.Bernhardt, M.Knoke, K.Ludwig. Mycoses, 47, 7, p.330, October 2004.
Evaluation of Human Esophageal Epithelium Permeability in Presence of Different Formulations Containing Hyaluronic Acid and Chondroitin Sulphate. Gaia Pellegatta, Marco Spadaccini, Laura Lamonaca, Vincenzo Craviotto, Ferdinando D’Amico, Laura Ceriotti, Marisa Meloni, Alessandro Repici. Medical Devices: Evidence and Research 2020.
In Vitro Modelling of Barrier Impairment Associated with Gastro-Oesophageal Reflux Disease (GERD).Marisa Meloni, Paolo Buratti, Francesco Carriero, Laura Ceriotti. Clinical and Experimental Gastroenterology 2021.
Protective Mechanisms of Liquid Formulations for Gastro-Oesophageal Reflux Disease in a Human Reconstructed Oesophageal Epithelium Model.. Laura Ceriotti, Paolo Buratti, Enrico Stefano Corazziari, Marisa Meloni. 2022.