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Permeability
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DESCRIPTION
Our 3D epidermal models show great similarities with human skin. They allow topical or systemic application of raw materials or finished products to evaluate their effects on human skin metabolic activities and barrier function.
Confocal Raman spectroscopy or LC/MSMS can be used for characterization.
MODELS
PROTOCOL PRINCIPLE
The purpose of this study is to determine the penetration of the test product after topical application on the reconstructed human epidermis models.
DETAILED ASSAY PROCEDURE
REFERENCES
Comparison of xenobiotic metabolizing enzyme activities in ex vivo human skin and reconstructed human skin models from Skinethic.
Eilstein J., Léreaux G., Budimir N., Hussler G., Wilkinson S., Duché D., Arch Toxicol, 88, 1681-1694, 2014 .
Skin concentration of topically applied substances in reconstructed human epidermis (RHE) compared with human skin using in vivo confocal Raman microscopy.
Fleischli FD., Morf F., Adlhart C., Chimia, 69, 147-151, 2015.
Simultaneous absorption of vitamins C and E from topical emulsions using reconstructed human epidermis as a skin model.
B. Rozman, M. Gasperlin, E. Tinois-Tessonneaud, F. Pirot, F. Falson. European Journal of Pharmaceutics and Biopharmaceutics, 72 : 69-75, 2009.