AUTHORS : Shikhverdieva A S Terskikh V V Vasiliev A V Vorotelyak E A 2002 Biology Bulletin 2002 Jan-Feb ;29 (1):24-30 Russian Acad Sci, NK Koltsov Dev Biol Inst, Moscow 117808, Russia. Vorotelyak, EA, Russian Acad Sci, NK Koltsov Dev Biol Inst, Ul Vavilova 26, Moscow 117808, Russia. Print Simulation of migration of human epidermal keratinocytes over three-dimensional collagen gel We developed a novel model for studying migration of keratinocyte colonies over 3D collagen gel. It was shown that keratinocytes previously cultured on plastic and then seeded on the surface of collagen gel proliferate more actively than the primary cells. The gel density could significantly affect the morphology and migration of keratinocyte colonies. Modification of collagen get by fibronectin-like protein or poly-L-lysine altered the morphometric parameters of colonies without significant changes in the velocity of migration.