Preclinical Evaluation of Tolerability of a Selective, Bacteriostatic, Locally Active Vaginal Formulation

Background: Polybactum (Effik International,Brussels,Belgium) is a vaginal mucoadhesive product (medical device) designed to forma film that acts as a mechanical barrier with the aim of inhibiting
colonization by specific pathogens. It contains polycarbophil, a bio adhesive agent, and lauryl glucoside (LG),a nonionic surfactant that reinforces the barrier effect through its tensioactive properties.

Objective: To assess the local safety profile, tolerability, and efficacy of Polybactum formulations.

Methods: The following studies were performed on 3 Polybactum formulations: 2 ovules (Type1:LG 0.04% and Type2: LG 0.1%) and 1 gel formulation. Bacteriologic tests assessing the effects on normal vaginal flora and pathogens; in vitro and in vivo tests designed to assess cytotoxicity, as well as irritant and sensitizing potentials; biocompatibility, barrier, residence time, and absorption tests using reconstituted human vaginal epithelium were performed.

Results: Polybactum is a selective bacteriostatic agent that is active against Streptococcus agalactiae and Gardnerella vaginalis while sparing normal vaginal flora; that is, Lactobacillus spp. It had no cytotoxic, irritant, and sensitizing effects nor did it impair barrier and fence functions of the vaginal epithelium.The Type 1 ovuleshowed film-forming properties in vitro. Finally, LG absorption through reconstituted human vaginal epithelium was negligible, ruling out the riskf or possible systemic toxicity.

Conclusions: This favorable preclinical profile is encouraging and supports clinical studies on Polybactum Type 1ovules for the prevention and management of recurring bacterial vaginosis.

 

In vitro test approach

2016 ELSEVIER
Effik Italia S.p.A.,Cinisello Balsamo, Italy & VitroScreen Srl, Milan, Italy & Studio Brugali Food & Chemical Consulting, Almenno S. Salvatore,Italy & Laboratory of Toxicology, DiSFeB, Università degli Studidi Milano, Milan, Italy