光毒性

光毒性检测

 

描述 

基于表皮模型或全皮模型，通过表面涂抹和系统暴露的方式，进行体外光毒性检测，从而对化学样品进行分类。

 

模型 

	EpiSkinTM 表皮模型（大孔径）
	SkinEthicTM RHE 表皮模型
	T-SkinTM 全皮模型

 

操作原则

EpiSkin^TM表皮表皮模型和全皮模型可用于体外皮肤光毒性检测，应遵循相应的操作规范。

 

详细测试步骤 

• SkinEthic^TM RHE 表皮模型光毒性测试方法：例如，F. -X. Bernard等发布的测试方法（2000年）
• EpiSkin^TM 表皮模型（大孔径）光毒性测试方法（点击下载）

 

参考文献

Development of a highly sensitive in vitro phototoxicity assay using the SkinEthic^TM reconstructed human epidermis. F.-X. Bernard, C. Barrault, A. Deguercy, B. De Wever, M. Rosdy. Cell Biology and Toxicology, 16: 391-400, 2000.

 

An assessment of the phototoxic hazard of a personal ingredient using in vitro assays. P.A. Jones, A.V. King, L.K. Earl, R.S. Lawrence. Toxicology In Vitro, 17: 471-480, 2003.

 

Assessment of the phototoxic potential of compounds and finished products using a human reconstructed epidermis. J. Medina, C. Elaesser, V. Picarles, O. Grenet, M. Kolopp, S.-D. Chibout, A. de Brugerolle de Fraissinette. In Vitro & Molecular Toxicology, 14 : 157-168, 2001.

 

The EpiSkin^TM phototoxicity assay (EPA): development of an in vitro tiered strategy using 17 reference chemicals to predict phototoxic potency. D. Lelièvre, P. Justine, F. Christiaens, N. Bonaventure, J. Coutet, L. Marrot, J. Cotovio. Toxicology In Vitro, 21 : 977-995, 2007.

 

A reconstituted human epidermis to assess cutaneous irritation, photoirritation, and photoprotection in vitro. R. Roguet, C. Cohen, R. Rougier. In vitro skin toxicology; Irritation, Phototoxicity, sensitization. A. Rougier, A.M. Goldberg, H.I. Maibach EDs. Mary Ann Liebert, Inc New York, 141-149, 1994.